Several relics of Ambrose are also preserved; his jaw bone is held at the Church of St Ambrose of Milan, Barlow Moor, Manchester; one of his hands is preserved at Stanbrook Abbey now at Wass, North Yorkshire,and another hand is at Mount Angel Abbey in St. Benedict, Oregon; and his skull is preserved on the stairwell at Wardley Hall in Worsley, the one time home of the Downes family, and now the home of the Catholic Bishop of Salford.
The church of St Ambrose of Milan at Barlow Moor is in the parish of his birthplace. It was founded in 1932, and is dedicated to St Ambrose of Milan but changed to St Ambrose Barlow at his canonisation. St Ambrose Barlow Roman Catholic church and primary school in Astley is also named after Ambrose.
Other schools named after the saint include The Barlow Roman Catholic High School in Didsbury, St Ambrose Barlow Roman Catholic High School in Swinton near Manchester, and St Ambrose Barlow Catholic High School in Netherton, Merseyside.